Figure 3.

Bovine herpesvirus type 1 (BHV-1) neutralization titre of sera from Toll-like receptor 9 (TLR9)^–/– and TLR9^+/+ mice immunized with 10 µg of pgD (plasmid vector pCAN1 expressing the full-length glycoprotein D protein from BHV-1 under the cytomegalovirus promoter) on day 1 and 4 weeks later. Two weeks after the secondary immunization, mouse sera were assayed for BHV-1 neutralization. Data presented are values for individual mice and the bar represents the group mean. n = 4 for TLR9^–/– mice and n = 5 for TLR9^+/+ mice. TLR9^–/– and TLR^+/+ versus control, P < 0·05, by one-way analysis of variance (anova) followed by Tukey's multiple comparison test.