Figure 5.

Luciferase gene expression in skin following the administration of plasmids encoding luciferase in Toll-like receptor 9 (TLR9)^–/– and TLR^+/+ mice. Plasmids encoding luciferase and containing different numbers of CpG motifs were administered intradermally and, 2 days later, skin samples were assessed for expression of the luciferase gene. Data represent luciferase expression for injection sites of individual mice and the bar represents the median. All plasmids induced significant gene expression versus naive tissue (P < 0·05), as determined using one-way analysis of variance (anova) followed by Tukey's multiple comparison test. There were no significant differences among the different plasmids or between TLR9^–/– and TLR^+/+ mice. LUs, light units.