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. 2004 Oct;113(2):224–233. doi: 10.1111/j.1365-2567.2004.01946.x

Figure 5.

Figure 5

Histamine induces TLR2 and TLR4 mRNA expression. HUVEC (1 × 106 cells) were allowed to adhere in each of the wells of a six-well culture plates for 18–20 hr. Cell monolayers were then incubated with histamine (10 μm) alone or histamine+diphenhydramine (DPH, 10 μm) for 2 hr. After the incubation, total RNA was isolated and subjected to RT-PCR using TLR2 and TLR4 primers. The amplified products were electrophoresed on a 2% agarose gel. GAPDH mRNA expression was used for normalization. The results presented are representatives of four independent experiments.