Table 1.
Frozen PBMC from five VL patients, studied in acute and healed phases of disease, were cultured for 24 hr in medium alone (–) and in the presence of LAg (+) (2 × 107 equivalent promastigotes). Afterwards, cells were treated with appropriate IS3-conjugated antibody (anti-CD4 or anti-CD8) and treated with Tdt-FITC staining. Data are expressed as mean percentage ± SE.
P < 0·05: significantly different from healed subjects. Percentage of PCD in five healthy un-stimulated control (CTR) PBMCs: CD4+ (4 ± 2); CD8+ (2 ± 1). Percentage of CD4+ T cells: (A) 35 ± 8; (H) 42 ± 5; (CTR) 44 ± 8. Percentage of CD8+ T cells: (A) 30 ± 7; (H) 33 ± 5; (CTR) 34 ± 6.