Table 2.

Evaluation of apoptosis percentage in CD4⁺ /CCR5⁺ and CD4⁺ CCR3⁺ T lymphocytes inside the total CD4⁺ CCR5⁺ and CD4⁺ CCR3⁺ T populations using PI method after TCR activation by Lag

	% apoptosis
	CD4+ /CCR5+		CD4+ /CCR3
	−	+	−	+
Acute (A)	10·0 ± 2	35·0 ± 8*	2·5 ± 0·8	1·5 ± 0·5
Healed (B)	7·5 ± 1	5·0 ± 1	2·0 ± 1·0	2·5 ± 1·0

Frozen PBMC from five VL patients, studied in acute and healed phases of disease, were cultured for 24 hr in medium alone (–) or in the presence of LAg (+) (2 × 10 ⁷ equivalent promastigotes). Cells were treated with IS3-conjugated antibody anti-CD4, with anti-CCR5 or anti-CCR3 FICT-conjugated antibodies and incubated with PI. Data are expressed as mean percentage ±SE.

^*P < 0·05: significant different from healed subjects.