Table 3.
Cytokines −(pg/ml) | A | B | ||
---|---|---|---|---|
+ | − | + | − | |
IFN-γ | 20 ± 3 | 12 ± 2* | 20 ± 4 | 60 ± 6† |
IL-10 | 25 ± 4 | 40 ± 9** | 23 ± 5 | 40 ± 3** |
IL-4 | 5 ± 2 | 13 ± 3** | 15 ± 2 | 20 ± 3 |
Frozen PBMC from five VL patients, studied in acute and healed phases of disease, were cultured for 24 hr in medium alone (–) or in the presence of LAg (+) (2 × 107 equivalent promastigotes). Supernatants were filtered (25 µm) and tested for cytokine contents. Cytokine production by frozen PBMC from five healthy controls: IFN-γ: 6 ± 2 pg/ml; IL-10: 4 ± 1 pg/ml; IL-4: 0·8 ± 0·2 pg/ml. Data are expressed as mean ± SE.
P < 0·01: significantly different from healed subjects.
P < 0·05: significantly different from unstimulated cultures.
P < 0·01: significantly different from unstimulated cultures.