Figure 5.

Blocking of CD11b expression does not influence CD8α^– DC migration in vitro. Nycodenz-enriched, bead-sorted CD8α^– mDC were treated with either blocking anti-CD11b or isotype control Ig for 30 min at 4°. After washing to remove excess mAb, DC were placed in the upper wells of resting or activated EC-layered Transwells® and allowed to migrate for either 4 hr (resting EC) or 2 hr (activated EC) in response to CCL19 (a) or CCL21 (b). Results are expressed as percentage of mAb-blocked DC migration/control Ig-blocked migration (± 1 SD) to denote the degree to which transmigration was inhibited in the presence of blocking antibody. Data are from one experiment representative of at least two performed.