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. 2004 Nov;113(3):392–400. doi: 10.1111/j.1365-2567.2004.01970.x

Figure 6.

Figure 6

Representative micrograph of DNA end-labelling (TUNEL) of tissue sections from rats after 7 and 14 days of GXM administration, and immunohistochemistry for GXM in cytospin preparations from rat Spm cells (14 days post-inoculation). (a) TUNEL labelling of lung sections from 7-day GXM-treated rats reveals positive staining (arrow) next to metachromatic areas of methylgreen (arrow heads). Magnification × 400. (b) TUNEL positive cells (arrows) and metachromatic zones (arrow heads) in lung sections from 14-day GXM-treated rats. Magnification × 400. (c, d) TUNEL-positive cells (arrows) in liver and spleen sections from 14-day GXM-treated rats. Magnification × 400. (e) GXM immunoreactivity within Spm cells with nuclei with morphological features of apoptosis (14 days post-inoculation). Magnification (1000×). Cytospin preparations were counterstained with haematoxylin. (f) Percentage of cells with morphological features of apoptosis in cytospin preparations of Spm cell from control and 14-day GXM-injected rats. Three animals per group were used and three slides from each animal were prepared. At least 200 cells were counted by light microscopy in each cytospin preparations. Each column shows the mean ± SEM and the graph is representative of two independent experiments. *P < 0·05.