Figure 2.

Phenotypic analyses of ovine lymphatic dendritic cells and the binding activity ovCTLA4_eEGFP by flow cytometry (a) Ovine DC obtained by pseudo-afferent cannulation of the prefemoral lymph node were stained with control isotype antibodies (open histograms) and a panel of cell-surface antigen markers (filled histograms). Each histogram is representative of at least three different experiments. (b) Human or ovine DC were incubated with ovCTLA4_eEGFP derived from recombinant adenovirus modified fibroblasts. The bound ovCTLA4_eEGFP was detected with an anti-GFP mAb as shown in the shaded histogram and the negative-isotype matched mAb (X63) represented by the solid line. The dotted line represents blockade of ovCTLA4_eEGFP binding with CTLA4-Ig. (c)Human DC were stained with anti-CD80 and -CD86 mAbs to confirm the presence of the CTLA-4 receptors. Flow cytometric analysis of CD80 and CD86 staining is represented by the shaded histogram with X63 represented by the unshaded histogram.