Figure 3.

Alloinhibitory properties of ovCTLA4_eEGFP. (a) Dose–response inhibition of a two-way ovine MLR by ovCTLA4_eEGFP. Responses are expressed as the percentage of inhibition relative to the untreated MLR. (b) Comparative alloinhibitory properties of ovCTLA4_eEGFP, huCTLA4_eEGFP and native EGFP. Fusion proteins were normalized to 5 µg/ml of EGFP and added to an ovine MLR. CsA was added at 100 ng/ml as an established inhibitory control. (c) Ovine DC were transfected with ADVovCTLA4_eEGFP or ADV-EGFP. Cell transduction efficiency is demonstrated as a dot-plot representation (FL-1 versus forward scatter). (d) DC modified with ADVovCTLA4_eEGFP were used as stimulators of ovine PBMC at ratios of 1:10 and 1:100. Unmodified DC and DC modified with ADV-EGFP were used as controls. Each treatment group consisted of triplicates and each graph is representative of three different experiments. *P < 0·05, unpaired Student's t-test.