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. 2004 Dec;113(4):491–498. doi: 10.1111/j.1365-2567.2004.01993.x

Figure 1.

Figure 1

Modulation of LPS-induced cytokine production from bmDCs pre-exposed to ES-62 in vitro. Bone marrow-derived DCs (bmDCs) were treated with 2 μg/ml ES-62 or were left untreated for 24 hr prior to stimulation with 1 μg/ml E. coli LPS for the times indicated or for 24 hr (b, c). IL-12 p40 mRNA levels were assessed by TaqMan real-time RT-PCR and are expressed as mean values relative to HPRT mRNA, with errors calculated according to the manufacturer's instructions (a). IL-12 p40 (p40 monomer and homodimer and p70 heterodimer) and TNF-α in culture supernatants were determined by ELISA (b, c). Data are presented as mean plus standard deviation. ***P < 0·005 compared to non-ES-62-treated samples. Results are representative of at least three experiments.