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. 2004 Dec;113(4):491–498. doi: 10.1111/j.1365-2567.2004.01993.x

Figure 2.

Figure 2

Modulation of bmDC and bmMφ cytokine production by in vivo exposure of bone marrow progenitors to ES-62. Mice were exposed to PBS or ES-62 (0·05 μg/hr or 0·2 μg/hr) by constant release from osmotic pumps for 2 weeks (a–g). The bmDCs were stimulated with 1 μg/ml E. coli LPS for 8 hr (a–d) or 24 hr (e, f). IL-12 p40 and p35, TNF-α and IL-10 mRNA levels (a–d) were assessed by TaqMan real-time RT-PCR and are expressed as mean values relative to HPRT mRNA. IL-12 p40 and IL-10 in 24-hr culture supernatants were assessed by ELISA (e, f). In bmMφs were stimulated with 100 U/ml IFN-γ and 100 ng/ml S. minnesota LPS for 24 hr. IL-12 p40 protein in culture supernatants was measured by ELISA. The ELISA data are presented as mean plus standard deviation; *P < 0·05, ***P < 0·005 compared to PBS treatment. In and bmMφs derived from mice exposed to Ova or ES-62 (0·2 μg/hr) by constant release from osmotic pumps for 2 weeks were stimulated with 100 U/ml IFN-γ and 100 ng/ml S. minnesota LPS for 8 hr. IL-12 p40 and p35 mRNA levels were assessed by TaqMan real-time RT-PCR and are expressed as mean values relative to HPRT mRNA. Results are representative of three experiments.