Table 2.
Effect of methyl-β-cyclodextrin (MCD) and 4-amino-5-(4-methylphenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine (PP1) on the polarization of human leucocyte antigen-A2 (HLA-A2) and other receptors on the surface of target cells induced by beads loaded with anti-intercellular adhesion molecule-1 (ICAM-1) monoclonal antibody (mAb)
| Polarization (% ± SD) | ||||||
|---|---|---|---|---|---|---|
| Bead type | Treatment | ICAM-1 | HLA-A2 | HLA-DR | TFR | CD45 |
| None | 80 ± 6* | 74 ± 7 | 12 ± 2 | 11 ± 2 | 8 ± 3 | |
| Anti-ICAM-1 | +MCD | ND | 31 ± 8 | ND | ND | ND |
| +PP1 | ND | 44 ± 5 | ND | ND | ND | |
*
Cell-surface ICAM-1 was stained with biotinylated CL203 mAb recognizing domain 4 of ICAM-1 molecules, while the beads were loaded with HB9580 mAb specific for domain 2 of ICAM-1.
ND, not determined; SD, standard deviation; TFR, transferrin receptor.