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. 2002 Jan;105(1):83–91. doi: 10.1046/j.0019-2805.2001.01339.x

Figure 3.

Figure 3

Increase in cell-associated TNF-R by chloroquine. U-937 cells were incubated with indicated amounts of chloroquine (CQ) or BB-3103 (BB) for 2 hr, and stimulated with PMA (10 ng/ml) for 24 hr. Cells were lysed in buffer containing 0·5% NP-40, protease inhibitors and 5 mm EDTA. The levels of soluble p55 TNF-R sTNF-R (a) and p75 sTNF-R (b) in the culture supernatant and cell-associated p55 TNF-R (c) and p75 TNF-R (d) in the cell lysates were measured by ELISA. Each value represents mean±SD (n = 3).