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Interleukin (IL)-4, IL-5, IL-13 and interferon-γ (IFN-γ) levels determined by enzyme-linked immunosorbent assay (ELISA) in spleen cell cultures prepared from recipients of IFN-γ gene knockout (gko) grafts and recipients of wild-type (wt) grafts. Spleens were harvested on days 1, 4, 8 and 22 postinduction. Error bars represent the standard error (SE) of the mean cytokine levels obtained from three individual mice in each group.
