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. 2002 Jan;105(1):63–72. doi: 10.1046/j.0019-2805.2001.01353.x

Figure 5.

Figure 5

Mean levels of antibodies to double-stranded DNA (anti-dsDNA) observed in interferon-γ (IFN-γ) gene knockout (gko) graft recipients on several days postinduction (a). Controls consisted of B6D2F1 mice (b) and recipients of wild-type grafts (c). Error bars represent the standard error (SE) of the mean anti-dsDNA levels obtained from three individual mice in each group. Significant differences [P < 0·05; analysis of variance (anova) followed by a Tukey's Multiple Comparison Test] between the levels observed for IFN-γ gko graft recipients and B6D2F1 control mice are indicated by an asterisk. Also shown is a representative standard curve, obtained by serial dilution of MRL/lpr sera, starting at a 1 : 4000 dilution. One unit of anti-dsDNA was defined in each experiment as the absorbance at 550 nm (A550) observed for MRL/lpr sera at a 1 : 4000 dilution. Sera from IFN-γ gko graft recipients produced A550 values in the linear portion of the standard curve at several dilutions of 1 : 50 or greater.