Figure 1.

Proliferation and differentiation of Mtb-PPD-specific T lymphocytes. Human lymphocytes were cultured with autologous DC and Mtb-PPD (autologous MLC). After 6 days, uptake of [³H]thymidine and release of Th1/Th2-associated cytokines were measured. The results of a representative autologous MLC are expressed as mean±SD of three replicates. The [³H]thymidine assay shows high proliferative activity in MLC when compared to controls (a). Cytokine release assays demonstrate that in MLC the production and secretion of IFN-γ, (b) but not of IL-5 or (c) of IL-10 (d) are increased.