Figure 5.

Characterization of reactivated T lymphocytes undergoing apoptosis. Mtb-PPD-specific human T lymphocytes were reactivated by 400 µg/ml Mtb-PPD. To characterise apoptotic T-cell populations, the TUNEL technique was combined with cell surface staining for different T-cell markers. Two-colour dot plots show a representative experiment with lymphocytes from a donor following reactivation by 400 µg/ml Mtb-PPD. Results indicate that CD3⁺, CD4⁺, CD8⁺, αβ⁺ as well as γδ⁺ cells undergo apoptosis.