Figure 4.

Co-culturing monocytes with CD3⁺ T cells restores CXCR4 expression to that observed with monocytes in PBMC cultures. Purified monocytes were cultured in medium (clear histograms and symbols) or with 10⁻⁷ m dexamethasone (filled histograms and symbols) for 48 hr. CXCR4 expression on monocytes in PBMC cultures was significantly increased compared with purified monocytes. Dexamethasone also significantly increased CXCR4 expression. Mean ± SEM of 10 experiments (a). Co-culture of purified monocytes with the increasing ratios of purified autologous CD3⁺ T cells for 48 hr resulted in increased CXCR4 expression. Mean ± SEM of six experiments (b).* denotes statistical significance at P < 0·05.