Table 2. Cytotoxicity induced by FasL-transfected cell lines or by PEL-CTL.
| % 51Cr-release at E:T | ||||
|---|---|---|---|---|
| Effector | Target | 10:1 | 5:1 | SR |
| NIH3T3.FasL* | LF+ | 70·0 | 64·1 | 18·0 |
| NIH3T3.FasL & Jo2 (2 µg/ml)* | LF+ | nm | 13·0 | 18·0 |
| NIH3T3.FasL* | LF− | 1·6 | 0·0 | 11·9 |
| NIH3T3† | LF+ | 0·0 | 0·0 | 18·0 |
| L1210.FasL† | LF+ | 23·8 | 21·4 | 18·0 |
| L1210.FasL† | LF− | 0·0 | 0·0 | 16·5 |
| BW.FasL† | LF+ | 68·3 | 60·0 | 18·0 |
| BW.Vector† | LF+ | 0·0 | 0·0 | 18·0 |
| BW.(wild-type)† | LF+ | 0·0 | 0·0 | 18·0 |
| BW.FasL† | LF− | 0·3 | 0·6 | 11·0 |
| P0 anti-LF+ PEL* | LF+ | 60·5 | 43·2 | 10·0 |
| P0 anti-LF+ PEL* | LF− | 6·4 | 2·1 | 20·6 |
Effector cells were mixed with
51
Cr-labelled targets (3 × 104 cells/well).
*
Lysis was measured after 4·5 hr.
†
Lysis was measured after 7 hr.
nm, not measured.