Table 4. Cytotoxicity induced by AKR PEL after preincubation with cognate FasL-expressing cells.
| % 51Cr release at E:T | |||||
|---|---|---|---|---|---|
| Effector | Target | Pre-incubated with | 5:1 | 2·5:1 | SR |
| AKR anti-L1210 PEL | L1210 | alone | 69·3 | 28·2 | 7·3 |
| AKR anti-L1210 PEL | L1210 | L1210.FasL (1:0·5) | 14·5 | 2·2 | 7·3 |
| AKR anti-L1210 PEL | L1210 | L1210.FasL (1:0·25) | 21·0 | 2·4 | 7·3 |
| AKR anti-L1210 PEL | L1210 | L1210.Vector (1:0·5) | 11·7 | 1·6 | 7·3 |
| AKR anti-L1210 PEL | L1210 | L1210.Vector (1:0·25) | 18·9 | 2·8 | 7·3 |
Four days after a secondary alloimmunization, AKR anti-L1210 PEL (k anti-d) were prepared and resuspended in DMEM supplemented with interleukin-2 (20 IU/ml). PEL were preincubated for 22 hr with FasL-transfected L1210.FasL or empty vector-transfected L1210.vector cells, prior to adding
51
Cr-labelled targets L1210 (3 × 10 4 cells/well). Lysis was measured after 3 hr at 37°. Ratio of AKR PEL to preincubation partners (L1210.FasL or L1210.Vector) during preincubation is indicated in parenthesis.