Table 5. CTL-mediated cytotoxicity against FasL-transfected targets.
| % 51Cr release at E:T | |||||||
|---|---|---|---|---|---|---|---|
| PEL-CTL | Target | 20:1 | 10:1 | 5:1 | 2·5:1 | 1·25:1 | SR |
| Allogeneic | |||||||
| AKR anti-L1210 | L1210.FasL | 65·2 | 49·6 | 28·6 | 8·1 | ||
| AKR anti-L1210 | L1210.Vector | 53·2 | 35·5 | 19·4 | 7·3 | ||
| BALB/c anti-BW | BW.FasL | 67·6 | 39·1 | nm | 13·8 | ||
| BALB.c anti-BW | BW (wild-type) | 57·1 | 35·5 | nm | 10·7 | ||
| Syngeneic | |||||||
| AKR anti-BW.Vector | BW.FasL | 36·5 | 15·2 | 8·7 | 17·4 | ||
| AKR anti-BW.Vector | BW.Vector | 25·5 | 12·0 | 8·5 | 21·2 | ||
| AKR splenocytes* | BW.FasL | 27·7 | 23·1 | 18·0 | 10·6 | ||
| AKR splenocytes* | BW.Vector | 17·4 | 16·4 | 14·3 | 11·7 | ||
Allogeneic AKR anti-L1210 PEL (k anti-d) and BALB/c anti-BW PEL (d anti-k), and syngeneic AKR PEL (with irradiated BW.Vector cells) were obtained 4 days after a second immunization
Splenocytes were derived from AKR mice immunized with irradiated BW.Vector tumour cells. Six days after a second immunization, splenocytes were suspended in RHFM medium and co-cultured wth 3000 Rad-irradiated and mitomycin C-treated BW.Vec (ratio was about 10:1). The concentration of interleukin-2 was 30 IU/ml. After 5 days the cultures were harvested and viable lymphocytes (effector cells) were separated by centrifugation on Ficoll-Metrizoate.
Cr-labelled target cells (3 × 104 cells/well); cytolytic assay lasted 3 hr for allogeniec effectors or 5·5 hr for syngeneic effectors. nm, not measured.