Figure 6.

Effect of the IDO inhibitor, 1-MT, on allogeneic PBL proliferation after coculture with HeLa vector control cells or CD80-expressing HeLa cell clones. Proliferation of PBL cocultured with MRC-5 vector control cells or HeLa cell clones, in the absence (open bars) or presence (grey bars) of exogenous IL-2, was determined after 5 days of coculture by measuring PBL uptake of ³H-thymidine. At initiation of coculture, 1-MT was added at 0 µm or 250 µm as indicated. Tritiated thymidine was added at 0·5 µCi/well for the final 18 hr of coculture. Data are representative of three independent experiments. Error bars represent the standard deviation of ³H-thymidine incorporation in triplicate cultures.