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. 2002 Apr;105(4):419–429. doi: 10.1046/j.1365-2567.2002.01389.x

Figure 1.

Figure 1

Binding of anti-human T-cell receptor (TCR) monoclonal antibody to purified naive mouse T cells in flow cytometric analyses. Cells were either single stained with phycoerythrin (PE)-conjugated antibodies (ordinate) or double stained with fluorescein isothiocyanate (FITC)- and PE-conjugated antibodies (abscissa) on various T-cell markers. Cells were labelled as follows, with the following antibodies. (a) Goat anti-human (GAH) PE conjugate. (b) Double labelled with anti-mouse CD4 FITC and anti-mouse CD8 PE. (c) Single labelled with anti-mouse CD3 PE. (d) Single labelled with anti-mouse Fas PE. (e) Double labelled with anti-mouse CD4 FITC and OR2 anti-human TCR with GAH PE conjugate. (f) Double labelled with anti-mouse CD8 FITC and OR2 anti-human TCR with GAH PE conjugate. (g) Double labelled with anti-mouse CD4 FITC and OR5 anti-human TCR with GAH PE conjugate. (h) Double labelled with anti-mouse CD8 FITC and OR5 anti-human TCR with GAH PE conjugate. (i) Double labelled with anti-mouse CD4 FITC and Syn 2H-11 anti-human TCR with GAH PE conjugate. (j) Double labelled with anti-mouse CD8 FITC and Syn 2H-11 anti-human TCR with GAH PE conjugate.