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. 2002 May;106(1):60–70. doi: 10.1046/j.1365-2567.2002.01397.x

Figure 7.

Figure 7

The delay in antigen presentation is not a result of its conjugation with Escherichia coli enterotoxin B subunit (EtxB). A20µWT cells were incubated with a predetermined stimulating dose of 40 µg/ml of phosphorylcholine (PC)-EtxB-ovalbumin (OVA) conjugate or medium alone (a). The specificity of targeting OVA to the B-cell receptor (BCR) was tested with an increasing concentration of either PC-G33D-OVA or G33D-OVA conjugate (b). Cells were incubated with the antigens for 0–240 min at 37°, washed and fixed with 1% paraformaldehyde (a), or incubated for 1 hr at 37° and washed (b). Following incubation with the antigens, DO.11 cells were added and incubated with the fixed antigen-presenting cells (APC) for 18–20 hr. Supernatants were analysed with interleukin-2 (IL-2)-responsive HT-2 cells. Data represent mean proliferation of quadruplicate samples±standard error of the mean (SEM). The data represent two similar independent experiments. c.p.m., counts per minute.