Figure 2.

Inhibition of LL-37-induced mast cell chemotaxis by LL-37 antiserum. Mast cells were allowed to migrate towards 5 µg/ml LL-37 after addition of LL-37 antiserum or rabbit preimmune control serum (1 : 500 dilution) to the chemoattractant in the lower compartment. Supernatants from the mast cell mixtures were obtained after incubation of mast cells with 5 µg/ml LL-37 for 3 hr at 37°, whereas the supernatants from the lower chamber were obtained by centrifuging the lower chamber contents after a 3-hr chemotaxis assay. These supernatants were further applied in the lower chamber and used as chemoattractants in the presence or absence of specific antiserum. Values are compared between the presence or absence of LL-37 antiserum. **P < 0·005. Each bar represents the mean±standard deviation (SD) of three separate experiments.