Figure 7.

Phenotype comparison of isolated primary-culture (left column) and conventional fourth-passage RA-SFB (right column) from one representative patient. Expression of Thy-1 (A, B), MHC-II (C, D), prolyl-4-hydroxylase (E, F), procollagen III (G, H), c-Fos (I, J), and c-Jun (K, L). While the percentages of cells positive for MHC-II (C) and the MFI for c-Jun (K) were significantly higher in isolated primary-culture RA-SFB, the percentages of cells positive for prolyl-4-hydroxylase (F), procollagen III (H), and c-Fos (J) were significantly higher in conventional fourth passage (see Table 5 for details). PE, phycoerythrine; FITC, fluoresceine isothiocyanate.