. 2000 Nov 21;3(1):72–76. doi: 10.1186/ar142

Table 5.

Phenotype of negatively isolated primary-culture rheumatoid arthritis (RA)-synovial fibroblasts (SFB) in comparison with fourth-passage RA-SFB

	Primary-culture		Fourth-passage		Fourth-passage
	RA-SFB		RA-SFB		RA-SFB
	Isolated cells		Isolated cells		Conventionally passaged cells
	(n = 9 patients)		(n = 3 patients)		(n = 6 patients)

Antigen	Percent	MFI	Percent	MFI	Percent	MFI
Thy-1 (CD90; mAb AS02)	73.8 ± 9.6	109 ± 20	86.6 ± 4.6	41 ± 7^#	85.9 ± 4.3	95 ± 17^x
MHC-I	^a77.2 ± 8.1	130 ± 54	99.2 ± 0.2^#	117 ± 48	97.4 ± 1.4^#	175 ± 78
MHC-II	^a65.8 ± 12.3	223 ± 84	4.7 ± 2.9^#	59 ± 25	4.8 ± 2.4^#	80 ± 21
Aminopeptidase N (CD13)	84.8 ± 5.9	444 ± 226	97.2 ± 1.6	108 ± 49	97.9 ± 1.2^#	236 ± 95
VCAM-1 (CD106)	^c17.5 ± 9.8	122 ± 42	n.d.	n.d.	^c0.6 ± 0.1	21 ± 5^#
Prolyl-4-hydroxylase	84.8 ± 2.9	164 ± 45	98.7 ± 0.8^#	164 ± 62	98.3 ± 0.7^#	189 ± 82
Vimentin	^a93.2 ± 1.5	592 ± 290	99.5 ± 0.4^#	717 ± 126	99.7^#	325
Procollagen I	^a44.8 ± 13.0	25 ± 2	92.7 ± 2.8^#	25 ± 12	91.0 ± 4.0^#	28 ± 5
Procollagen III	^a50.0 ± 14.3	26 ± 3	94.1 ± 1.8^#	21 ± 12	90.2 ± 5.2^#	22 ± 4
c-Fos	^a57.4 ± 14.0	20 ± 2	63.5 ± 16.4	10 ± 5	95.0 ± 0.6^#x	17 ± 2
c-Jun	^b42.5 ± 14.0	21 ± 5	n.d.	n.d.	^d42.1 ± 12.0	9 ± 2^#
Jun-D	^a53.0 ± 14.8	21 ± 4	68.1 ± 13.2	11 ± 5	93.0 ± 1.3^#x	18 ± 3

Data presented as means ± SEM. MFI, Mean fluorescence intensity; mAb, monoclonal antibody; MHC, major histocompatibility complex; VCAM-1, vascular adhesion molecule-1; n.d., not determined. For vimentin expression in conventionally passaged fourth-passage RA-SFB, the median is shown because of a large deviation from the mean. Significant differences between groups are indicated in bold. ^#P ≤ 0.05 Mann–Whitney U test versus primary-culture RA-SFB. ^xP ≤ 0.05 Mann–Whitney U test versus fourth-passage RA-SFB (isolated cells). ^an = 7 RA patients. ^bn = 6 RA patients. ^cn = 5 RA patients. ^dn = 4 RA patients.