Skip to main content
. 2002 May;106(1):53–59. doi: 10.1046/j.1365-2567.2002.01404.x

Figure 1.

Figure 1

Induction of CD36 protein and mRNA in THP-1 cells. Cells were cultured for 48 hr in the compounds as indicated or solvent control (Con). CD36 surface protein was measured by flow cytometry (a). In control cells (a, top left), the filled area indicates isotope control immunoglobulin as fluorescence background; the unfilled area represents specific staining with anti-CD36 monoclonal antibody. Cell surface staining of CD36 for all other treatment conditions is represented graphically by the filled areas. There were no significant differences in isotype control staining between treatment conditions. CD36 mRNA levels were assessed by RT-PCR (b) using CD36 and GAPDH primers. GAPDH mRNA expression was used as an internal control for normalization purposes. (c) represents the mean±SE CD36/GAPDH mRNA levels of at least three independent experiments; * indicates significant differences as compared to the solvent control while ** indicates significance between combination treatment and treatment with RA or 15d-PGJ2 alone (P < 0·05).