Figure 1.

Cytofluorimetric analysis of IL-12Rβ2 subunit expression on T cells. Monocytes (1×10⁴) were incubated with acapsular C. neoformans (7698) plus or minus GXM (250 µg/ml) or encapsulated C. neoformans (6995) in the presence or absence of mAb to GXM (10 µg/ml). After 2 hr the monolayers were washed to remove unbound yeasts and autologous T cells (1×10⁵) were added. After 5 days of incubation T cells were harvested and IL-12Rβ2 subunit expression on the T-cell surface was analysed by cytofluorimeter. Control samples were treated with isotype control antibody (irrelevant mAb) and a FITC-conjugated anti-rat IgG. The upper panel shows an experiment representative of four others performed with similar results. The percentage of positive cells for IL-12Rβ2 expression is shown on each dot plot. The lower panel shows the results (mean± SEM) of four separate experiments from four different donors. Statistical significance for the indicated pairs of data was determined by use of Student's paired t-test.