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. 2002 Jun;106(2):200–211. doi: 10.1046/j.1365-2567.2002.01414.x

Figure 2.

Figure 2

Disruption of the lytB gene increases the γδ T-cell stimulatory potential of Escherichia coli. (a) Vγ9+ T-cell outgrowth in the presence of bacterial extracts. Peripheral blood mononuclear cells (PBMC) were incubated over a period of 6 days with medium alone, or with low molecular weight extracts prepared from wild-type E. coli (black triangles), ΔgcpE (open circles), and ΔlytB (open squares), at different dilutions. Data shown represent mean values+standard error of the mean from three donors analysed. (b) Flow cytometry analysis. PBMC were incubated with medium alone (top left panel), or with LMW extracts prepared from WT E. coli (top right panel), ΔgcpE (bottom left panel), or ΔlytB (bottom right panel), at a dilution of 1 : 12 500. Panels shown are representative data obtained from one blood donor.