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. 2002 Jun;106(2):200–211. doi: 10.1046/j.1365-2567.2002.01414.x

Figure 5.

Figure 5

Fractionation of bacterial extracts by anion-exchange chromatography. ΔgcpE and ΔlytB low molecular weight (LMW) extracts, respectively, were passed over a reverse phase (RP) column and fractionated on a Source 15Q column, using an ammonium formiate gradient (20–500 mm). Absorbance at 280 nm is shown as a solid line for ΔlytB and a dashed line for ΔgcpE. Fractions collected were first tested in pools consisting of four each; fractions 19 to 22 were subsequently tested individually for their ability to upregulate CD69 expression on Vδ2+ T cells. Samples tested were: medium control; isopentenyl pyrophosphate (IPP) as positive control, 10 µm; 3-kDa LMW filtrate, 1 in 50 000 (LMW) extract; LMW extract after RP column chromatography (=sample loaded onto a Source 15Q column), 1 in 50 000 (RP); Source 15Q flow-through, 1 in 50 000 (f/t); Source 15Q wash fraction, 1 in 50 000; fractions 11–35, in pools or individually, 1 in 400 000. Solid bars, ΔgcpE; hatched bars, ΔlytB.