Figure 2.
Irradiated A20-HL cells up-regulate CD80 expression on untreated A20-HL cells. Irradiated A20-HL cells alone (5 × 105 cells) (a), untreated and BCECF-labelled A20-HL cells alone (2·5 × 105 cell/ml)(b), or the mixture of them (c)were incubated for 48 hr. In (d), irradiated A20-HL cells were isolated from BCECF-labelled A20-HL cells by the membrane filter in Millicell inserts and incubated. Then, these cells were examined for the CD80 expression by staining with anti-CD80 mAb (16-10A1, hamster IgG) and phycoerythrin-conjugated anti-hamster IgG antibody.