Figure 2.

NKT cells are activated by culture with GM-CSF or cross-linking CD3–T-cell antigen receptor complexes. Morphological transformation of NKT cells in response to GM-CSF or CD3 cross-linking with mAb (OKT3). Cells were cultured for 3 days. Proliferation and cytokine responses of NKT cells activated by antibody cross-linking of CD3–TCR complexes. NKT cells were cultured for 3 days in plastic wells coated with anti-CD3 antibody and containing irradiated monocytes from the same donor. Cell proliferation was measured (mean c.p.m.±SEM) by incorporation of [³H]thymidine and cytokines released by enzyme-linked immunosorbent assay. NKT cells activated with anti-CD3 antibody, closed bars; untreated NKT cells, open bars.