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. 2002 Aug;106(4):496–502. doi: 10.1046/j.1365-2567.2002.01451.x

Figure 4.

Figure 4

CD4+ T cells are the major cell-type responsive to HQ. Mice were injected in the footpad with KLH in alum. Seven days afterward, draining lymph node cells were incubated in vitro with anti-CD8 or anti-CD4 mAbs on ice for 30 min, followed by incubation with complement at 37° for 45 min After washing, cells from each treatment group were stimulated with KLH (50 µg/ml) in the presence of HQ (20 or 50 µm) for 4 days and IL-4 levels in the culture supernatants were analysed by ELISA. The data represent the means±SEM (n=3). *P<0·01, relative to a group treated with complement only.