Figure 4.

Confocal microscopy of the foot-and-mouth disease virus (FMDV) non-structural 2C antigen in pulmonary macrophages (Pul-Mφ) [(a), (b)], and the virion antigen detected with 4C9 antibody in either Pul-Mφ[(c), (d)] or BHK-21 cells [(e), (f)], at 6 hr postinfection with FMDV O₁ Lausanne. The cells were either untreated [(a), (c) and (e)] or treated with 100 µg/ml cycloheximide [(b), (d) and (f)] for the duration of the experiment. Actin microfilaments were stained (red) using Alexa 546 phalloidin. The small pictures to the left, to the right and below the main picture in (a), (b), (c) and (d) are sectional views of the left-hand and right-hand cells shown in the main picture.