Figure 2.

Expression of G1 cell cycle regulatory proteins in interleukin-2 (IL-2)-stimulated Th1 cells. (a) Lysates were prepared from resting (R) T helper 1 (Th1) cells, or from Th1 cells stimulated for 24, 48, or 72 hr with IL-2. Expression of the various G1 cell-cycle regulatory proteins was assessed by immunoblotting. Equal loading of cell extracts was confirmed using an antibody (Ab) that recognized actin. (b). Lysates were prepared from resting Th1 cells, or from Th1 cells stimulated for 24, 48, or 72 hr with IL-2. Expression of p21^Cip1 and p27^Kip1 was assessed by immunoblotting. Densitometric analysis was performed, and the results are presented as percentage actin. This experiment was been repeated twice with similar results obtained on each occasion.