Figure 3.

Effects of n-butyrate on cyclin-dependent kinase inhibitors (CDKIs) in interleukin-2 (IL-2)-stimulated T helper 1 (Th1) cells. Th1 cells were incubated in primary cultures containing IL-2+n-butyrate, or antigen (Ag)+n-butyrate, with or without IL-2. After 24 hr, the Th1 cells were isolated and lysates were prepared. p21^Cip1 and p27^Kip1 were immunoprecipitated, and the expression of p21^Cip1 and p27^Kip1, as well as their association with cyclin-dependent kinase 4 (cdk4), was assessed by immunoblotting. This experiment was been repeated, with similar results obtained on each occasion.