Figure 5.

Expression of G1 cell-cycle regulatory proteins in anergic and non-anergic T helper 1 (Th1) cells following restimulation with antigen (Ag). Th1 cells were incubated for 48 hr in primary culture with interleukin-2 (IL-2)+n-butyrate, or with Ag+IL-2+n-butyrate. The Th1 cells were then restimulated with Ag for 24 hr, at which time they were lysed. Expression of cyclin-dependent kinase (cdk)2, cdk4, cdk6, cyclin D2, cyclin D3, and cyclin E was assessed by immunoblotting. Equal loading of protein was confirmed by staining with an anti-actin antibody.