Figure 3.

Assessment of the specificity of anti-Id responses for private Id determinants. Binding to each patient's Id immunoglobulin (left column) represents reactivity with determinants on the whole variable region (arbitrarily 100%). The presence of 50% normal human serum blocks reactivity with public determinants, leaving only antibodies against private Id determinants (right column). In each case, there was evidence for reactivity against private Id expressed by natural Id immunoglobulin, demonstrating optimal folding of the scFv expressed in vivo.