Deposition of C-components of classic and alternative pathways on P1- and PLD-treated erythrocytes. Erythrocytes incubated with buffer (Control), P1 or PLD (5 µg/ml) were exposed to C8d-HS, incubated with polyclonal sera against C1q, C3, C4, or fB, and submitted to flow cytometry analysis. Results are representative of three different experiments carried out in duplicate and represented as mean±SD.