Figure 3.

Loxosceles venom P1 toxin incorporates into erythrocytes and causes loss of expression of glycophorins. Erythrocytes were treated with buffer, P1 (•) or PLD (○), and analysed for the expression of GPA and GPC by flow cytometry using the antibodies Bric 256 (GPA) and Bric 4 (GPC). The ability of the toxins P1 and PLD to bind to the erythrocyte surface was analysed using monospecific polyclonal rabbit serum against P1 (anti-F35) or monospecific mouse serum against PLD (anti-PLD), respectively. Results are representative for three different experiments expressed as mean of duplicates ±SD.