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. 2002 Sep;107(1):93–101. doi: 10.1046/j.1365-2567.2002.01483.x

Figure 4.

Figure 4

L. intermedia venom P1 toxin, but not PLD, induces cleavage of GPA and GPC on erythrocyte membranes. Erythrocytes were incubated with buffer, P1, or PLD. Erythrocyte ghosts were prepared and run on 12% SDS–PAGE under non-reducing conditions and Western blotted. Blots were probed with monoclonal antibodies recognizing different glycophorin epitopes: (a) Bric256: GPA extracellular epitope; (b) Bric163: GPA intracellular epitope; (c) Bric4: GPC extracellular epitope; (d) BGRL100: GPC intracellular epitope.