Figure 1.

Flow cytometric analysis of human CD45, CD8 and CD4 T-cell populations present in the spleen from grafted NOD-scid mice treated with human IL-18 (a) and human IL-12 (b). Three NOD-scid mice transplanted with human PBL were given the control (PBS), IL-18 (62·5 ng), and IL-18 (250 ng), or control, IL-12 (62·5 ng) and IL-12 (250 ng/mouse). Human PBL in spleen cells were analysed 3 weeks after the last administration for human CD45⁺ single and CD4⁺ CD8⁺ double staining. The analyses were performed on gated lymphocytes with FSC/SSC characteristics. Lymphocytes can be distinguished in the quadrant analysis. The number in the upper left and lower right quadrants are the percentages of CD8⁺ CD4⁻ T cells and CD4⁺ CD8⁻ T cells, respectively. The histograms show CD45 single-stained lymphocytes, while the numbers in each histogram indicate the per cent of gated cells. The vertical axis varied from maxima of 10 to 200 for the analysis of CD45⁺ cells, because there was interexperimental variation in compatibility of human lymphocytes from a donor to NOD-scid mice from a single litter. Data shown are representative of four independent experiments.