Figure 3.

Induction of apoptosis in spleen human CD4⁺ and human CD8⁺ T cells from human PBL–NOD-scid mice treated with PBS, human IL-18 alone, human IL-12 alone, or IL-18 + IL-12. Flow cytometric analyses of annexin V⁺ human CD4⁺ and CD8⁺ T cells are presented. Human PBL–NOD-scid mice were given PBS and cytokines (250 ng/mouse). Analyses were performed on gated lymphocytes with FSC/SSC characteristics. Furthermore, PI⁻ CD4⁺ and PI⁻ CD8⁺ T cells were also gated on the quadrants. The lymphocytes (dots) can be distinguished in the quadrant analyses of PI and annexin V. The number in each quadrant indicates differentiation markers. Data are representative of three independent experiments with similar results obtained in each experiment.