Figure 4.

Effects of human IL-12 on human IL-18-dependent up-regulation of human CD4⁺ and CD8⁺ T cells from the peritoneal cavity in human PBL–NOD-scid mice. The histogram shows CD45 single-stained lymphocytes. The number in each histogram indicates the per cent of gated cells. Analyses were performed on gated lymphocytes with FSC/SCC characteristics. Flow cytometric analyses of annexin V⁺ human CD8⁺ T cells. Analyses were performed on gated PI⁻ human CD8⁺ T cells. The lymphocytes can be distinguished in the lower right analysis quadrants of PI and annexin V. Numbers in the quadrants indicate the per cent of gated cells expressing the indicated markers. Data are representative of three independent experiments with similar results obtained in each.