Figure 5.

Effects of anti-human IFN-γ mAb on human IL-18-dependent up-regulation and apoptotic cell induction of human CD45⁺ cells, and CD4⁺, and CD8⁺ T cells. Flow cytometric analyses of these cells present in the spleen from grafted NOD-scid mice treated with PBS, IL-18 (250 ng) + anti-IFN-γ mAb (1/100 dilution), and IL-18 (250 ng) + anti-IFN-γ mAb (1/50 dilution) were performed. The proportion (a) and population (b) of the cells were analysed on gated lymphocytes with FSC/SCC characteristics. Results are expressed as means ±SEM of three independent assays. (c) Annexin V⁺ hu-CD4⁺ or CD8⁺ T cells as determined by FACS analyses performed on gated PI⁻ human CD4⁺ or CD8⁺ T cells. Lymphocytes can be distinguished in the quadrants of PI and annexin V. The number in the lower right quadrant indicates the per cent of gated cells expressing the indicated markers. Asterisks denote significant differences vs. IL-18 (*P < 0·05). Data are representative of three independent experiments with similar results obtained in each.