Figure 2.
Effect of medium fetal bovine serum concentration on the regulation of STAT-1 protein in the presence of LPS/TNF-α and RA. THP-1 cells were plated in RPMI-1640 medium with different concentrations of FBS. Cells were pretreated with RA (10−8 or 10−6 m as indicated) for 16 hr and then LPS and TNF-α were added to cells with fresh RA for anther 48 hr of incubation. (a) Western blot analysis showing the induction of STAT-1 protein with 10 and 3% FBS in the presence or absence of 10−8 m RA. Bar graph shows results from two experiments (control value for each group set to 1·0). (b) Analysis of Western blot data showing the regulation of STAT-1 protein by LPS/TNF-α and RA (10−8 and 10−6 m) in the presence of 10% FBS. Means ±SD from two experiments.