Figure 5.

Ovine dendritic cells (DCs) rapidly take up soluble antigen by macropinocytosis and receptor-mediated endocytosis. Fluorescence was quantified by flow cytometry. Cells were incubated either at 0° (□) or 37° (▪) prior to and during the experiments. (a) DCs and lucifer yellow; (b) peripheral blood mononuclear cells (PBMCs) and lucifer yellow; (c) DCs and fluorescein isothiocyanate (FITC)-dextran; (d) PBMCs and FITC-dextran. Bar graphs represent the mean fluorescence of the markers taken up by ovine in vitro-derived DCs or PBMCs.