Table 1. [3H]Thymidine uptake of adherent peripheral blood mononuclear cell (PBMC) cultures during dendritic cell (DC) generation demonstrated that proliferation was not present in our DC cultures.
Stimulation index | ||||
---|---|---|---|---|
Exp No. | Day 0 | Day 2 | Day 4 | Day 6 |
1 | ND | 1·5 | 1·8 | 1·8 |
2 | ND | 2·0 | 1·6 | 1·5 |
Results are given as stimulation indices (SI) of the c.p.m. of non-irradiated cells ÷ the c.p.m. of irradiated cells.
An SI of >2 indicates proliferation.
c.p.m., counts/min; ND, not done.